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For reverse transfection, 100 pM of each RNAi in 500 l OptiMEM, plus 5 l Lipofectamine RNAiMAX (Invitrogen) was added to wells

One has to keep in mind that leptin takes part in the pathophysiology of both asthma and obesity and that outcomes of these diseases may overlap, especially in the context of lung function [156,192,193,194]

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